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rsa 1  (Promega)

 
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    Promega rsa 1
    Rsa 1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rsa 1/product/Promega
    Average 90 stars, based on 1 article reviews
    rsa 1 - by Bioz Stars, 2026-03
    90/100 stars

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    Data are represented as group mean optical density values (OD 450 ) determined using the IDEXX Q fever antibody ELISA. Anti- Coxiella antibody measured in a 1:1,000 dilution of serum collected from guinea pigs at day 0, day 21 (filled shapes) and 42 (open shapes) post-inoculation with C . burnetii Nine Mile. Day 0 antibody levels were similar to saline controls. Data are presented by route of inoculation (intraperitoneal i.p.; intranasal i.n.; saline controls). Error bars represent standard deviations. Group mean OD 450 were statistically different from saline controls (one way ANOVA with Tukey’s multiple comparison test; ***P = <0.0001).

    Journal: PLoS ONE

    Article Title: Standardized guinea pig model for Q fever vaccine reactogenicity

    doi: 10.1371/journal.pone.0205882

    Figure Lengend Snippet: Data are represented as group mean optical density values (OD 450 ) determined using the IDEXX Q fever antibody ELISA. Anti- Coxiella antibody measured in a 1:1,000 dilution of serum collected from guinea pigs at day 0, day 21 (filled shapes) and 42 (open shapes) post-inoculation with C . burnetii Nine Mile. Day 0 antibody levels were similar to saline controls. Data are presented by route of inoculation (intraperitoneal i.p.; intranasal i.n.; saline controls). Error bars represent standard deviations. Group mean OD 450 were statistically different from saline controls (one way ANOVA with Tukey’s multiple comparison test; ***P = <0.0001).

    Article Snippet: C . burnetii Nine Mile strain ( Cb 9M) phase 1 RSA 411 (lot 3662550) was obtained from BEI Resources (Manassas, VA, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Saline, Comparison

    Data are represented as group mean (n = 8) by route of exposure to C . burnetii Nine Mile ( Cb 9M, i.p. intraperitoneal; i.n. intranasal; saline controls). Error bars represent standard deviation; boxes denote 25 th and 75 th quartiles. Group mean body weights of saline and i.p. inoculated groups were statistically different at day 7 p.i. (one-way ANOVA with Tukey’s multiple comparison test; *P = 0.003).

    Journal: PLoS ONE

    Article Title: Standardized guinea pig model for Q fever vaccine reactogenicity

    doi: 10.1371/journal.pone.0205882

    Figure Lengend Snippet: Data are represented as group mean (n = 8) by route of exposure to C . burnetii Nine Mile ( Cb 9M, i.p. intraperitoneal; i.n. intranasal; saline controls). Error bars represent standard deviation; boxes denote 25 th and 75 th quartiles. Group mean body weights of saline and i.p. inoculated groups were statistically different at day 7 p.i. (one-way ANOVA with Tukey’s multiple comparison test; *P = 0.003).

    Article Snippet: C . burnetii Nine Mile strain ( Cb 9M) phase 1 RSA 411 (lot 3662550) was obtained from BEI Resources (Manassas, VA, USA).

    Techniques: Saline, Standard Deviation, Comparison

    Data are shown as the zone of erythema (mm) at intradermal vaccine injection sites challenged at day 42 p.i., grouped by route of sensitization with C . burnetii Nine Mile ( Cb 9M) (i.p., intraperitoneal; i.n., intranasal) or saline controls. Measurements are presented for terminally harvested injection sites at day 7 p.c. (open shapes) and day 21 p.c. (filled shapes). Values are presented by challenge dose (A) 0 μg Coxevac, (B) 1 μg Coxevac, (C) 2 μg Coxevac, and (D) 4 μg Coxevac. 1 Histopathological scoring (0–5) is as noted in . Error bars represent group mean and standard deviations. Group mean differences assessed by one-way ANOVA with Tukey’s multiple correction test; *P≤0.05.

    Journal: PLoS ONE

    Article Title: Standardized guinea pig model for Q fever vaccine reactogenicity

    doi: 10.1371/journal.pone.0205882

    Figure Lengend Snippet: Data are shown as the zone of erythema (mm) at intradermal vaccine injection sites challenged at day 42 p.i., grouped by route of sensitization with C . burnetii Nine Mile ( Cb 9M) (i.p., intraperitoneal; i.n., intranasal) or saline controls. Measurements are presented for terminally harvested injection sites at day 7 p.c. (open shapes) and day 21 p.c. (filled shapes). Values are presented by challenge dose (A) 0 μg Coxevac, (B) 1 μg Coxevac, (C) 2 μg Coxevac, and (D) 4 μg Coxevac. 1 Histopathological scoring (0–5) is as noted in . Error bars represent group mean and standard deviations. Group mean differences assessed by one-way ANOVA with Tukey’s multiple correction test; *P≤0.05.

    Article Snippet: C . burnetii Nine Mile strain ( Cb 9M) phase 1 RSA 411 (lot 3662550) was obtained from BEI Resources (Manassas, VA, USA).

    Techniques: Injection, Saline

    Images are representative of guinea pigs inoculated i.n. with C . burnetii (C and D) or unsensitized (saline) (A and B), then challenged with a 2 μg intradermal dose of vaccine (Coxevac) at day 42 p.i.. A) Inflammation at day 7 p.c. in unsensitized guinea pig is multifocal (arrows) and limited to the deep dermis. B) Inflammation at day 21 p.c. in unsensitized guinea pig is similar to day 7 (arrow). C) More severe inflammation with extension through the dermis (arrows) at day 7 p.c. in Cb 9M-sensitized guinea pig. D) Progression of inflammation in Cb 9M-sensitized guinea pig at day 21 p.c. (arrows) with increased frequency of neutrophils and presence of microabscesses (arrowheads).

    Journal: PLoS ONE

    Article Title: Standardized guinea pig model for Q fever vaccine reactogenicity

    doi: 10.1371/journal.pone.0205882

    Figure Lengend Snippet: Images are representative of guinea pigs inoculated i.n. with C . burnetii (C and D) or unsensitized (saline) (A and B), then challenged with a 2 μg intradermal dose of vaccine (Coxevac) at day 42 p.i.. A) Inflammation at day 7 p.c. in unsensitized guinea pig is multifocal (arrows) and limited to the deep dermis. B) Inflammation at day 21 p.c. in unsensitized guinea pig is similar to day 7 (arrow). C) More severe inflammation with extension through the dermis (arrows) at day 7 p.c. in Cb 9M-sensitized guinea pig. D) Progression of inflammation in Cb 9M-sensitized guinea pig at day 21 p.c. (arrows) with increased frequency of neutrophils and presence of microabscesses (arrowheads).

    Article Snippet: C . burnetii Nine Mile strain ( Cb 9M) phase 1 RSA 411 (lot 3662550) was obtained from BEI Resources (Manassas, VA, USA).

    Techniques: Saline

    Data are presented as histological scores at termination, following day 42 p.i. intradermal challenge, grouped by route of sensitization with C . burnetii Nine Mile ( Cb 9M) (i.p., intraperitoneal; i.n., intranasal) or saline controls. Scores are shown for injection sites harvested at day 7 p.c. (open shapes) and day 21 p.c. (filled shapes). Values are presented by challenge dose (A) 0 μg Coxevac, (B) 1 μg Coxevac, (C) 2 μg Coxevac, and (D) 4 μg Coxevac. 1 Histopathological scoring (0–5) is as noted in . Error bars represent group mean and standard deviations. Group mean differences assessed by one-way ANOVA with Tukey’s multiple correction test; *P≤0.05; ** P≤0.01.

    Journal: PLoS ONE

    Article Title: Standardized guinea pig model for Q fever vaccine reactogenicity

    doi: 10.1371/journal.pone.0205882

    Figure Lengend Snippet: Data are presented as histological scores at termination, following day 42 p.i. intradermal challenge, grouped by route of sensitization with C . burnetii Nine Mile ( Cb 9M) (i.p., intraperitoneal; i.n., intranasal) or saline controls. Scores are shown for injection sites harvested at day 7 p.c. (open shapes) and day 21 p.c. (filled shapes). Values are presented by challenge dose (A) 0 μg Coxevac, (B) 1 μg Coxevac, (C) 2 μg Coxevac, and (D) 4 μg Coxevac. 1 Histopathological scoring (0–5) is as noted in . Error bars represent group mean and standard deviations. Group mean differences assessed by one-way ANOVA with Tukey’s multiple correction test; *P≤0.05; ** P≤0.01.

    Article Snippet: C . burnetii Nine Mile strain ( Cb 9M) phase 1 RSA 411 (lot 3662550) was obtained from BEI Resources (Manassas, VA, USA).

    Techniques: Saline, Injection

    Data are presented for each inoculation group (saline (n = 4), Cb 9M: C . burnetii Nine Mile, i.n.: intranasal, (n = 9)). Coxevac challenge (2 μg delivered i.d.) was performed at day 42 p.i. (A) Histopathological scoring (0–5) is as noted in , (B) zone of erythema (mm). Error bars represent group mean and standard deviations. **Group means were statistically different for both histological scores and erythema (P value 0.002, 0.002 respectively, assessed by one-sided t test).

    Journal: PLoS ONE

    Article Title: Standardized guinea pig model for Q fever vaccine reactogenicity

    doi: 10.1371/journal.pone.0205882

    Figure Lengend Snippet: Data are presented for each inoculation group (saline (n = 4), Cb 9M: C . burnetii Nine Mile, i.n.: intranasal, (n = 9)). Coxevac challenge (2 μg delivered i.d.) was performed at day 42 p.i. (A) Histopathological scoring (0–5) is as noted in , (B) zone of erythema (mm). Error bars represent group mean and standard deviations. **Group means were statistically different for both histological scores and erythema (P value 0.002, 0.002 respectively, assessed by one-sided t test).

    Article Snippet: C . burnetii Nine Mile strain ( Cb 9M) phase 1 RSA 411 (lot 3662550) was obtained from BEI Resources (Manassas, VA, USA).

    Techniques: Saline